Through analysis of the MS/MS spectrum of 1 and its minor derivative, stieleriacine E ( 2), it was possible to assign the structure of 2 without isolation. Its biosynthesis was proposed based on an in silico gene cluster analysis. The structure of this compound, named stieleriacine D ( 1), was elucidated utilizing HRESIMS, 1D- and 2D-NMR data as a new N-acylated dehydrotyrosine derivative. HPLC-MS analysis of culture broth obtained from strain Enr13 T revealed the presence of lipophilic metabolites, of which the major compound was isolated by preparative reversed-phase HPLC. Phylogenetic tree reconstruction based on 16S rRNA gene sequences and multi-locus sequence analysis supports the delineation of strain Enr13 T from characterized species part of the phylum of Planctomycetes. biofilm obtained from seawater sediment close to Panarea Island, Italy.
Here, we describe the isolation and characterization of the planctomycetal strain Enr13 T isolated from a Posidonia sp. 6Central Facility for Microscopy, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germanyīacteria of the phylum Planctomycetes occur ubiquitously in marine environments and play important roles in the marine nitrogen- and carbon cycle, for example as scavengers after phototrophic blooms.5Department of Microbial Interactions, Institute of Microbiology, Friedrich Schiller University Jena, Jena, Germany.4Department of Microbiology, Radboud University, Nijmegen, Netherlands.3Leibniz Institute DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.2German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Braunschweig, Germany.
1Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.Birthe Sandargo 1,2†, Olga Jeske 3†, Christian Boedeker 3, Sandra Wiegand 4, Jan-Peer Wennrich 1,2, Nicolai Kallscheuer 4, Mareike Jogler 5, Manfred Rohde 6, Christian Jogler 4,5* and Frank Surup 1,2*
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